Tag Archives: RNAi

Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

lncRNA

Long non-coding RNAs (lncRNAs) represent a large subgroup of RNAs that are over 200 nucleotides long and have a limited protein-coding potential. They play an important role in diverse cellular processes by regulating the gene expression at transcriptional or post-transcriptional levels. This ability of lncRNAs to modulate gene expression renders them indispensable for normal development CRISPRs (Clustered Regularly Interspaced Palindromic ...

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Knockdown of Nuclear-Located Enhancer RNAs and Long ncRNAs Using Locked Nucleic Acid GapmeRs

lncRNA

The human genome is widely transcribed outside of protein-coding genes, producing thousands of noncoding RNAs from different subfamilies including enhancer RNAs. Functional studies to determine the role of individual genes are challenging with noncoding RNAs appearing to be more difficult to knockdown than mRNAs. One factor that may have hindered progress is that the majority of noncoding RNAs are thought ...

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Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

lncRNA

Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of action. One method to discern cellular function is by selective knockdown of a specific lncRNA species. However, achieving consistent knockdown has proven to be ...

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Upcoming Webinar – Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

lncRNA

Date: Tuesday, August 25, 2015 Time: Session 1: 9:00–10:00 am CDT (UTC-5) Session 2: 1:00–2:00 pm CDT (UTC-5) Presenter: Kim Lennox, MSc Program overview Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of ...

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Sheena Saayman, Scripps, to Speak on lncRNAs at the Non-Coding RNAs and RNAi Conference

Sheena Saayman, The Scripps Research Institute, will speak about HIV-encoded antisense lncRNA at the Non-Coding RNAs and RNAi Research & Therapeutics Conference, to be held June 19-20, 2014 in San Diego, CA. Long non-coding RNAs (lncRNAs) have a wide range of roles in human cells, including their function as epigenetic modulators, which plays a pivotal role in the regulation of ...

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RNAi 2014: Short & Long Non-coding RNAs

9th International Conference & Exhibition 25-27 March 2014 St Hilda’s College, Oxford, UK The 9th Annual Oxford RNAi Conference, RNAi2014, will discuss short and long non-coding RNAs, their roles in gene regulation, functional studies, RNA biomarkers and their therapeutic applications. Considerable attention will be given to chemical design and in vitro and in vivo delivery strategies for small RNAs. Key topics to be covered include: ...

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RNAi Screens and the Reagents That Enable Them

from Genetic Engineering News 2013 (Vol. 33, No. 19) by Jeanene Swanson RNAi screens for functional genomics typically look for loss- or gain-of-function phenotypes. They currently have many applications, including target discovery and validation, lead identification and optimization, mechanism of action discovery, predictive toxicology, and biomarker identification. While reagents are gaining ground, technical difficulties remain in how best to perform ...

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Perspectives on the mechanism of transcriptional regulation by long non-coding RNAs

Long non-coding RNAs (lncRNAs) are increasingly being recognized as epigenetic regulators of gene transcription. The diversity and complexity of lncRNA genes means that they exert their regulatory effects by a variety of mechanisms. Although there is still much to be learned about the mechanism of lncRNA function, general principles are starting to emerge. In particular, the application of high throughput ...

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