Tag Archives: qRT-PCR

Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) may function as a potential biomarker for glioma

lncRNA

Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, researchers from Nanchang University used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. They found that expression of CCAT2 was up-regulated in glioma ...

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A practical qPCR approach to detect TERRA, the elusive telomeric repeat-containing lncRNA

lncRNA

Telomeres, the heterochromatic structures that protect the ends of the chromosomes, are transcribed into a class of long non-coding RNAs, telomeric repeat-containing RNAs (TERRA), whose transcriptional regulation and functions are not well understood. The identification of TERRA adds a novel level of structural and functional complexity at telomeres, opening up a new field of research. TERRA molecules are expressed at ...

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Profiling of human long non-coding RNAs with CaptureSeq

Researchers from the Garvan Institute of Medical Research compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This ...

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Function of lncRNAs and approaches to lncRNA-protein interactions

lncRNA

Long non-coding RNAs (lncRNAs), which represent a new frontier in molecular biology, play important roles in regulating gene expression at epigenetic, transcriptional and post-transcriptional levels. More and more lncRNAs have been found to play important roles in normal cell physiological activities, and participate in the development of varieties of tumors and other diseases. Previously, we have only been able to ...

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