Tag Archives: knockdown

Effective knockdown of Drosophila long noncoding RNAs by CRISPR interference.

lncRNA

Ji-Long Liu (Univ Oxford) presents ‘Effective knockdown of Drosophila long noncoding RNAs by CRISPR interference.’ A presentation at the ‘Techniques & Resources’ session of the Annual Drosophila Research Conference at The Allied Genetics Conference, July 13-17, 2016, Orlando, Florida. Read the abstract: http://www.genetics-gsa.org/genetics/… For more presentations from #TAGC16 visit: http://www.genetics-gsa.org/tagcvideos or browse the playlists at: https://www.youtube.com/user/Genetics… More on #TAGC16: http://www.genetics2016.org/ ...

Read More »

Knockdown of Nuclear-Located Enhancer RNAs and Long ncRNAs Using Locked Nucleic Acid GapmeRs

lncRNA

The human genome is widely transcribed outside of protein-coding genes, producing thousands of noncoding RNAs from different subfamilies including enhancer RNAs. Functional studies to determine the role of individual genes are challenging with noncoding RNAs appearing to be more difficult to knockdown than mRNAs. One factor that may have hindered progress is that the majority of noncoding RNAs are thought ...

Read More »

Knockdown of long noncoding RNAs in breast cancer

Traditionally genetics has held a protein centric view with RNA seen as an intermediate step between DNA and protein. Recently, the emerging evidence of pervasive transcription throughout the genome has challenged this view1,2. Long noncoding RNAs (lncRNA) are selectively expressed during different cell cycles3 as well as transcribed differently in specific cell types4, which emphasizes their importance in regulating cell ...

Read More »

Webinar – lncRNA function and the use of Antisense LNA™ GapmeRs for knockdown of RNA

lncRNA

Watch our recent webinar and hear Dr. Zhen Xing from Liuqing Yang’s lab at MD Anderson Cancer Center present their work on long non-coding RNAs (lncRNA). In a recent Cell paper, they showed that knockdown of BCAR4 significantly reduced breast cancer metastasis in a mouse model. Hear about their approaches for the analysis of lncRNA function and get helpful tips. ...

Read More »

Upcoming Webinar – Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

lncRNA

Date: Tuesday, August 25, 2015 Time: Session 1: 9:00–10:00 am CDT (UTC-5) Session 2: 1:00–2:00 pm CDT (UTC-5) Presenter: Kim Lennox, MSc Program overview Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of ...

Read More »

LncRNA2Target – a database for differentially expressed genes after lncRNA knockdown or overexpression

lncRNA

Long noncoding RNAs (lncRNAs) have been emerged as critical regulators of gene expression at epigenetic, transcriptional and post-transcriptional level, yet what genes are regulated by lncRNAs remains to be characterized. To assess the effects of a specific lncRNA on gene expression, increasing researchers profiled the genome-wide or individual gene expression level changes after knocking down or overexpressing the lncRNA. However, ...

Read More »