Tag Archives: immunoprecipitation

Urb-RIP – An Adaptable and Efficient Approach for Immunoprecipitation of lncRNAs and Associated RNAs/Proteins

Post-transcriptional regulation of gene expression is an important process that is mediated by interactions between mRNAs and RNA binding proteins (RBP), non-coding RNAs (ncRNA) or ribonucleoproteins (RNP). Key to the study of post-transcriptional regulation of mRNAs and the function of ncRNAs such as long non-coding RNAs (lncRNAs) is an understanding of what factors are interacting with these transcripts. While several ...

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A Dis3l2-mediated quality control pathway for lncRNA RMRP and other noncoding RNAs


Mutations in the 3′-5′ exonuclease DIS3L2 are associated with Perlman syndrome and hypersusceptibility to Wilms tumorigenesis. Previously, Harvard Medical School researchers found that Dis3l2 specifically recognizes and degrades uridylated pre-let-7 microRNA. However, the widespread relevance of Dis3l2-mediated decay of uridylated substrates remains unknown. Here, the researchers applied an unbiased RNA immunoprecipitation strategy to identify Dis3l2 targets in mouse embryonic stem ...

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