Tag Archives: discovery

lncScore – alignment-free identification of long noncoding RNA from assembled novel transcripts

lncRNA

RNA-Seq based transcriptome assembly has been widely used to identify novel lncRNAs. However, the best-performing transcript reconstruction methods merely identified 21% of full-length protein-coding transcripts from H. sapiens. Those partial-length protein-coding transcripts are more likely to be classified as lncRNAs due to their incomplete CDS, leading to higher false positive rate for lncRNA identification. Furthermore, potential sequencing or assembly error ...

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ExInAtor – driver lncRNA discovery using mutational patterns in tumour DNA

lncRNA

Long noncoding RNAs (lncRNAs) represent a vast unexplored genetic space that may hold missing drivers of tumourigenesis, but few such “driver lncRNAs” are known. Until now, they have been discovered through changes in expression, leading to problems in distinguishing between causative roles and passenger effects. Researchers at the Barcelona Institute of Science and Technology have developed a different approach for ...

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Discovery and annotation of long noncoding RNAs

lncRNA

Recent advances in RNA-sequencing technologies have led to the discovery of thousands of previously unannotated noncoding transcripts, including many long noncoding RNAs (lncRNAs) whose functions remain largely unknown. Discussed here are considerations and best practices in lncRNA identification and annotation, which we hope will foster functional and mechanistic exploration. Perhaps the biggest surprise of the postgenomic era has been the ...

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