Tag Archives: CRISPR-Cas9

Functional dissection of NEAT1 using genome editing

lncRNA

Large numbers of long noncoding RNAs have been discovered in recent years, but only a few have been characterized. NEAT1 (nuclear paraspeckle assembly transcript 1) is a mammalian long noncoding RNA that is important for the reproductive physiology of mice, cancer development, and the formation of subnuclear bodies termed paraspeckles. The two major isoforms of NEAT1 (3.7 kb NEAT1_1 and ...

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DECKO – Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs

lncRNA

For some time, scientists have been seeking a more efficient and reliable way to edit the genome and modify it to suit any need. A technique called CRISPR-Cas9 was recently launched as the solution to this problem, and has since taken a position as one of the most revolutionary techniques in molecular biology. Although CRISPR-Cas9 is much more powerful than ...

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