Tag Archives: chromatin

Scientists have identified a non-coding RNA, called Paupar, influences how healthy brains develop during early life. They have shown that Paupar orchestrates proteins that control neurodevelopment...

We are recruiting a talented, skilled, mature and dedicated postdoctoral fellow to undertake ambitious and challenging projects in the area of chromosome/chromatin biology...

Most DNA in the genomes of higher organisms does not code for proteins. RNA Polymerase II (Pol II) transcribes non-coding DNA into long non-coding RNAs (lncRNAs), but biological roles of lncRNA are unclear. Researchers at the University of Copenhagen found that mutations in the yeast lncRNA CUT60 result in poor growth. Defective termination of CUT60 transcription causes read-through transcription across the ...

CIMA Institute in Spain is recruiting highly motivated and talented postdoctoral scientist to study the molecular mechanisms of long noncoding RNAs in chromatin replication and cancer...

The telomere protects against genomic instability by minimizing the accelerated end resection of the genetic material, a phenomenon that results in severe chromosome instability that could favor the transformation of a cell by enabling the emergence of tumor-promoting mutations. Some mechanisms that avoid this fate, such as capping and loop formation, have been very well characterized; however, telomeric non-coding transcripts, ...

Dietary interventions dramatically affect metabolic disease and lifespan in various aging models. Here, researchers from the Shanghai Institutes for Biological Sciences profiled liver microRNA (miRNA), coding, and long non-coding RNA (lncRNA) expression by high-throughput deep sequencing in mice across multiple energy intake and expenditure interventions. Strikingly, three dietary intervention network design patterns were uncovered: (1) lifespan-extending interventions largely repressed the ...

Research Topic – Roles of Long Non-Coding RNAs in Embryonic Development and Cellular Homeostasis Advances in genomics over the past two decades have established that more than two thirds of the eukaryotic genome is pervasively transcribed, while just >2% codes for protein. Intriguingly, the degree of organismal complexity appears proportional to the transcribed non-coding portion of the genome. The majority ...

Recent studies have uncovered thousands of long non-coding RNAs (lncRNAs) in human pancreatic β cells. β cell lncRNAs are often cell type specific and exhibit dynamic regulation during differentiation or upon changing glucose concentrations. Although these features hint at a role of lncRNAs in β cell gene regulation and diabetes, the function of β cell lncRNAs remains largely unknown. In ...

Long intergenic non-coding RNAs (lincRNAs) are defined as RNA transcripts that are longer than 200 nucleotides. By definition, these RNAs must not have open reading frames that encode proteins. Many of these transcripts are encoded by RNA polymerase II, are spliced, and are poly-adenylated. This final fact indicates that there is a trove of information about lincRNAs in databases such ...

Capture Hybridization Analysis of RNA Targets (CHART) has recently been developed to map the genome-wide binding profile of chromatin-associated RNAs. This protocol uses a small number of 22-28 nucleotide biotinylated antisense oligonucleotides, complementary to regions of the target RNA that are accessible for hybridization, to purify RNAs from a cross-linked chromatin extract. RNA-chromatin complexes are next immobilized on beads, washed, ...