Tag Archives: antisense oligo

Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

lncRNA

The CRISPR/Cas9 system provides a revolutionary genome editing tool for all areas of molecular biology. In long non-coding RNA (lncRNA) research, the Cas9 nuclease can delete lncRNA genes or introduce RNA-destabilizing elements into their locus. The nuclease-deficient dCas9 mutant retains its RNA-dependent DNA-binding activity and can modulate gene expression when fused to transcriptional repressor or activator domains. Here, researchers from ...

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Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

lncRNA

Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of action. One method to discern cellular function is by selective knockdown of a specific lncRNA species. However, achieving consistent knockdown has proven to be ...

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Upcoming Webinar – Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

lncRNA

Date: Tuesday, August 25, 2015 Time: Session 1: 9:00–10:00 am CDT (UTC-5) Session 2: 1:00–2:00 pm CDT (UTC-5) Presenter: Kim Lennox, MSc Program overview Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of ...

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