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PV Nazarov*, T Kaoma, A Muller, S Fritah, L Vallar
*Corresponding author: PV Nazarov
Genomics Research Unit, CRP-Sante, Luxembourg, Luxembourg
F1000Posters 2013, 4: 1446 (poster) [English] – Poster [1.22 MB]
Presented at:
Benelux Bioinformatics Conference 2013 , 9 – 10 Dec 2013, P82
Affiliated Society:
Benelux Bioinformatics Conference
Background / Purpose:
We compared two experimental approaches – standard microarray technology and next-generation RNA-seq for the analysis of changes in lncRNA expression profiles in NSCLC cancer tissue samples.
We compared two experimental approaches – standard microarray technology and next-generation RNA-seq for the analysis of changes in lncRNA expression profiles in NSCLC cancer tissue samples.
Main conclusion:
Generally speaking, RNA-seq technique outperforms arrays when studying lncRNAs. Cufflinks status should be taken into account together with counts or FPKM values in order to avoid misinterpreting the results.
Generally speaking, RNA-seq technique outperforms arrays when studying lncRNAs. Cufflinks status should be taken into account together with counts or FPKM values in order to avoid misinterpreting the results.
Next steps:
Correlation between co-localized lncRNAs and mRNAs should be further investigated.
Correlation between co-localized lncRNAs and mRNAs should be further investigated.
Disclosures:
No relevant competing interests disclosed.
No relevant competing interests disclosed.
Funding:
Luxembourg FNR CORE grant, ASTSTRO
Luxembourg FNR CORE grant, ASTSTRO
Please note that most posters on this site present work that is preliminary in nature and has not been peer reviewed.
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