Long non-coding RNAs (lncRNA) represent an assorted class of transcripts having little or no protein coding capacity and have recently gained importance for their function as regulators of gene expression. Molecular studies on lncRNA have uncovered multifaceted interactions with protein coding genes. It has been suggested that lncRNAs are an additional layer of regulatory switches involved in gene regulation during development and disease. LncRNAs expressing in specific tissues or cell types during adult stages can have potential roles in form, function, maintenance and repair of tissues and organs.
Researchers from CSIR Institute of Genomics and Integrative Biology, India used RNA sequencing followed by computational analysis to identify tissue restricted lncRNA transcript signatures from five different tissues of adult zebrafish. The present study reports 442 predicted lncRNA transcripts from adult zebrafish tissues out of which 419 were novel lncRNA transcripts. Of these, 77 lncRNAs show predominant tissue restricted expression across the five major tissues investigated. Adult zebrafish brain expressed the largest number of tissue restricted lncRNA transcripts followed by cardiovascular tissue. The researchers also validated the tissue restricted expression of a subset of lncRNAs using independent methods. These data constitute a useful genomic resource towards understanding the expression of lncRNAs in various tissues in adult zebrafish. This study is thus a starting point and opens a way towards discovering new molecular interactions of gene expression within the specific adult tissues in the context of maintenance of organ form and function.
- Kaushik K, Leonard VE, KV S, Lalwani MK, Jalali S, et al. (2013) Dynamic Expression of Long Non-Coding RNAs (lncRNAs) in Adult Zebrafish. PLoS ONE 8(12), e83616. [article]