Monthly Archives: November 2016

Long noncoding RNA PVT1 may be a novel noninvasive biomarker for early diagnosis of cervical cancer

lncRNA

Eighty-eight cervical cancer patients, 64 cervical intraepithelial neoplasia patients, 25 breast cancer patients, 25 ovarian cancer patients, and 111 healthy control subjects were enrolled into this study. Researchers at Jiangxi University of Traditional Chinese Medicine measured PVT1 serum level in these participants and PVT1 expression in 20 pairs of cervical cancer tissues and adjacent paired normal tissues by quantitative reverse ...

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Humira treatment restores dysregulated lncRNA gene networks

lncRNA

from Psoriasis News Today by Joana Fernandes – Researchers identified abnormal genetic pathways associated with psoriasis, some of which may be restored after treatment with Humira (adalimumab), according to a new study published in the journal BMC Genomics. In the study, “Network Analysis Of Psoriasis Reveals Biological Pathways And Roles For Coding And Long Non-Coding RNAs,” researchers observed that many pathways associated ...

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Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

lncRNA

RNA fluorescence in situ hybridization (FISH), long an indispensable tool for the detection and localization of RNA, is becoming an increasingly important complement to other gene expression analysis methods. Especially important for long noncoding RNAs (lncRNAs), RNA FISH adds the ability to distinguish between primary and mature lncRNA transcripts and thus to segregate the site of synthesis from the site ...

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Circulating lncRNA levels too low to serve as biomarkers

lncRNA

Long non-coding RNAs (lncRNA) are a new class of regulatory molecules with diverse cellular functions. Recent reports have suggested that extracellular lncRNAs are detectable in human plasma and may serve as biomarkers. Here, researchers at the Ottawa Hospital Research Institute sought to investigate circulating lncRNAs as potential biomarkers for pulmonary arterial hypertension (PAH). Eighty-four lncRNAs, representing some of the most ...

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SINEUP lncRNAs represent a new versatile tool to improve the production of secreted proteins in biomanufacturing processes

lncRNA

Mammalian cells are an indispensable tool for the production of recombinant proteins in contexts where function depends on post-translational modifications. Among them, Chinese Hamster Ovary (CHO) cells are the primary factories for the production of therapeutic proteins, including monoclonal antibodies (MAbs). To improve expression and stability, several methodologies have been adopted, including methods based on media formulation, selective pressure and ...

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Exosomal long noncoding RNA CRNDE-h as a novel serum-based biomarker for diagnosis and prognosis of colorectal cancer

Cancer-secreted long non-coding RNAs (lncRNAs) are emerging mediators of cancer-host cross talk. Researchers at Shandong University aimed to illustrate the clinical significance of the lncRNA CRNDE-h in exosomes purified from the serum of patients with colorectal cancer (CRC). The study was divided into four parts: The exosome isolated methods and lncRNA detected methods which accurately and reproducibly measure CRC-related exosomal ...

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Retroposition as a source of antisense long non-coding RNAs with possible regulatory functions

lncRNA

Long non-coding RNAs (lncRNAs) are a class of intensely studied, yet enigmatic molecules that make up a substantial portion of the human transcriptome. In this work, researchers from Adam Mickiewicz University in Poznan link the origins and functions of some lncRNAs to retroposition, a process resulting in the creation of intronless copies (retrocopies) of the so-called parental genes. They found ...

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Long non-coding RNAs – spatial amplifiers that control nuclear structure and gene expression

Over the past decade, it has become clear that mammalian genomes encode thousands of long non-coding RNAs (lncRNAs), many of which are now implicated in diverse biological processes. Recent work studying the molecular mechanisms of several key examples – including Xist, which orchestrates X chromosome inactivation – has provided new insights into how lncRNAs can control cellular functions by acting ...

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A potential role of LncRNAs expression in cardiac cell proliferation

lncRNA

Long non-coding RNAs (LncRNAs) have been identified to play important roles in epigenetic processes that underpin organogenesis. However, the role of LncRNAs in the regulation of transition from fetal to adult life of human heart has not been evaluated. Researchers at Wenzhou Medical University used immunofiuorescent staining to determine the extent of cardiac cell proliferation. Human LncRNA microarrays were applied ...

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lncRNAs Neat1 and SAMMSON: similar, yet different anti-cancer targets

lncRNA

A team of researchers led by Chris Marine (VIB-KU Leuven) has shown that NEAT1, a long non-coding RNA, plays an important role in the survival of highly dividing cells – cancer cells in particular. The research has a striking similarity to earlier findings by the Marine lab about SAMMSON, a long non-coding RNA gene on which the growth of aggressive ...

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