Time: Session 1: 9:00–10:00 am CDT (UTC-5)
Session 2: 1:00–2:00 pm CDT (UTC-5)
Presenter: Kim Lennox, MSc
Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of action. One method to discern cellular function is by selective knockdown of a specific lncRNA species. However, achieving consistent knockdown has proven to be more challenging for lncRNAs than for mRNAs or miRNAs. In this webinar, we will discuss some of the issues encountered with lncRNA research. We will cover antisense oligonucleotide (ASO) and small interfering RNA (siRNA) methods for lncRNA knockdown. And, we will show how cellular localization of a specific lncRNA target informs the choice of knockdown method.
About the Presenter
Kim Lennox has been a research scientist in the Molecular Genetics group at IDT for 13 years. She studied genetics at Iowa State University and received an MSc in Biomolecular Archaeology from the University of Manchester (UK). Kim is an expert at using synthetic oligonucleotides to suppress gene expression and is a co-author on 17 scientific papers describing various methods of gene knockdown. Her recent publications describe optimization of antisense oligonucleotides and siRNAs to degrade mRNAs and long non-coding RNAs (lncRNAs). She also has published work characterizing steric blocking antisense oligonucleotides, such as anti-miRNA oligonucleotides (AMOs) and splice-switching oligonucleotides (SSOs).