Tag Archives: lncrna

Structural and Functional Annotation of Long Noncoding RNAs

lncrna

Protein-coding RNAs represent only a small fraction of the transcriptional output in higher eukaryotes. The remaining RNA species encompass a broad range of molecular functions and regulatory roles, a consequence of the structural polyvalence of RNA polymers. Albeit several classes of small noncoding RNAs are relatively well characterized, the accessibility of affordable high-throughput sequencing is generating a wealth of novel, ...

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Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

lncRNA

RNA fluorescence in situ hybridization (FISH), long an indispensable tool for the detection and localization of RNA, is becoming an increasingly important complement to other gene expression analysis methods. Especially important for long noncoding RNAs (lncRNAs), RNA FISH adds the ability to distinguish between primary and mature lncRNA transcripts and thus to segregate the site of synthesis from the site ...

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SINEUP lncRNAs represent a new versatile tool to improve the production of secreted proteins in biomanufacturing processes

lncRNA

Mammalian cells are an indispensable tool for the production of recombinant proteins in contexts where function depends on post-translational modifications. Among them, Chinese Hamster Ovary (CHO) cells are the primary factories for the production of therapeutic proteins, including monoclonal antibodies (MAbs). To improve expression and stability, several methodologies have been adopted, including methods based on media formulation, selective pressure and ...

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Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) may function as a potential biomarker for glioma

lncRNA

Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, researchers from Nanchang University used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. They found that expression of CCAT2 was up-regulated in glioma ...

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Analysis of lncRNA MATLAT1 by In Situ Hybridization and Real-Time PCR

lncRNA

Long non-coding RNAs (lncRNAs) are important for transcription and for epigenetic or posttranscriptional regulation of gene expression and may contribute to carcinogenesis. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), an lncRNA involved in the regulation of the cell cycle, cell proliferation, and cell migration, is known to be deregulated in multiple cancers. Here, researchers from the University of Wisconsin School of Medicine ...

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DIGIT – a conserved lncRNA regulator of endoderm differentiation in embryonic stem cells

lncRNA

Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here, researchers from Massachusetts General Hospital combined genome-wide mapping of SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation of human embryonic stem cells (hESCs). They found that DIGIT is divergent to Goosecoid (GSC) and expressed during endoderm differentiation. Deletion of the SMAD3-occupied enhancer ...

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An artificial lncRNA targeting multiple miRNAs overcomes sorafenib resistance in hepatocellular carcinoma cells

lncRNA

Sorafenib resistance remains a major obstacle for the effective treatment of hepatocellular carcinoma (HCC), and a number of miRNAs contribute to this resistance. However, the regulatory networks of miRNAs are very complex, thus inhibiting a single miRNA may sequentially activate other compensatory pathways. In the present study, researchers at Harbin Medical University generated an artificial long non-coding RNA (AlncRNA), which simultaneously ...

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Transcriptome analyses identifies long noncoding RNAs regulating energy metabolism

lncRNA

  Genome-wide screens define 359 metabolically sensitive lncRNAs in key metabolic organs LncRNA functions were predicted by mRNA-lncRNA correlation analyses In vitro assays connect lncRNAs to specific metabolic and signaling pathways SREBP1c induces Gm16551 to activate an lncRNA-mediated negative feedback loop       To systemically identify long noncoding RNAs (lncRNAs) regulating energy metabolism, researchers from the National Heart, Lung and ...

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LINC00520 plays a functional role in breast cancer

lncRNA

Long non-coding RNAs (lncRNAs) have been implicated in normal cellular homeostasis as well as pathophysiological conditions, including cancer. Harvard Medical School researchers performed global gene expression profiling of mammary epithelial cells transformed by oncogenic v-Src, and identified a large subset of uncharacterized lncRNAs potentially involved in breast cancer development. Specifically, their analysis revealed a novel lncRNA, LINC00520 that is upregulated ...

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Comprehensive characterization of lncRNA-mRNA related ceRNA network across 12 major cancers

lncRNA

Recent studies indicate that long noncoding RNAs (lncRNAs) can act as competing endogenous RNAs (ceRNAs) to indirectly regulate mRNAs through shared microRNAs, which represents a novel layer of RNA crosstalk and plays critical roles in the development of tumor. However, the global regulation landscape and characterization of these lncRNA related ceRNA crosstalk in cancers is still largely unknown. Here, researchers ...

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