Tag Archives: lncrna
Long non-coding RNAs (lncRNA) which are longer than 200 base pairs in length, play important role in the cellular machinery. Chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) are neoplasms of B-cells.
Researchers at Istanbul University aimed to investigate circulating lncRNA levels of CLL and MM patients. For this purpose they selected 5 candidate lncRNAs (TUG1, LincRNA-p21, MALAT1, HOTAIR, and GAS5) where the first two are regulated by p53. Analyses were performed by real-time PCR using cDNA synthesized from plasma RNAs. In both disease groups differential levels of plasma lncRNAs were observed. LincRNA-p21 was the only molecule displaying significant changes in the CLL group while all remaining lncRNAs showed significant differences in the MM group. In the MM group only TUG1 showed higher levels than the healthy volunteers. In conclusion, the expression levels of the candidate lncRNA molecules display a general trend for tissue- and disease-specific expression which can provide important potential biomarkers specific to the particular disease type.
- Isin M, Ozgur E, Cetin G, Erten N, Aktan M, Gezer U, Dalay N. (2014) Investigation of Circulating LncRNAs in B-cell Neoplasms. Clin Chim Acta [Epub ahead of print]. [abstract]
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Natural antisense transcripts (NATs) exist ubiquitously as pivotal molecules to regulate coding gene expression. Sirtuin 1 (Sirt1) is a NAD-dependent deacetylase which is involved in myogenesis. However, whether Sirt1 transcribes NAT during C2C12 differentiation is still unknown. In this study, researchers at Northwest A&F University, China identified a Sirt1 NAT which was designated as Sirt1 antisense long non-coding RNA (AS lncRNA) by sequencing and bioinformatic analysis. The level of Sirt1 AS lncRNA was greater in spleen but less in muscle tissue. The expression of both Sirt1 mRNA and Sirt1 AS lncRNA decreased during C2C12 myogenic differentiation, whereas the levels of miR-34a, which targets Sirt1, increased gradually. They further found that the half-life of Sirt1 AS lncRNA was 10h, but that of Sirt1 mRNA was 6h in C2C12 cells treated with 2μg/ml Actinomycin D. Therefore, compared with Sirt1 mRNA, Sirt1 AS lncRNA was more stable. Overexpression of Sirt1 AS lncRNA increased the levels of Sirt1 protein, whereas overexpression of Sirt1 AS lncRNA mutant did not affect the level of Sirt1 protein in C2C12 cells. Moreover, downregulation of Sirt1 mRNA caused by miR-34a was counteracted by Sirt1 AS lncRNA in C2C12 cells.
- Wang Y, Pang WJ, Wei N, Xiong Y, Wu WJ, Zhao CZ, Shen QW, Yang GS. (2014) Identification, stability and expression of Sirt1 antisense long non-coding RNA. Gene [Epub ahead of print]. [abstract]
Post Doctoral Scientist for studying Long non-coding RNA (lncRNA) biology in Cardiovascular Development and Regeneration
Posted: January 14, 2014
Expires: February 28, 2014
Requisition number: LncRNA, Cardiovascular development, Stem cells, Development, RNA biology, Regeneration
Science jobs from University of Cologne:
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Biologists have studied the functionality of a poorly understood category of genes, which produce long non-coding RNA molecules rather than proteins. Some of these genes have been conserved throughout evolution, and are present in 11 species ranging from man to frog. The research was lead at the University of Lausanne, in partnership with EPFL and the Swiss Institute of Bioinformatics (SIB -SIB). It has been published today in Nature.
Gliomas are the most lethal type of primary brain tumor in adult. Long noncoding RNAs (lncRNAs), which are involved in the progression of various cancers, may offer a potential gene therapy target in glioma.
Researchers at the First Affiliated Hospital of Nanjing Medical University, China first classified gliomas into three molecular subtypes (namely LncR1, LncR2 and LncR3) in Rembrandt dataset using consensus clustering. Survival analysis indicated that LncR3 had the best prognosis, while the LncR1 subtype showed the poorest overall survival rate. The results were further validated in an independent glioma dataset GSE16011. Additionally, they collected and merged data of the two databases (Rembrandt and GSE16011 dataset) and analyzed prognosis of each subtype in WHO II, III and IV gliomas. The similar results were obtained. Gene Set Variation Analysis (GSVA) demonstrated that LncR1 subtype enriched cultured astroglia’s gene signature, while LncR2 subtype was characterized by neuronal gene signature. Oligodendrocytic was rich in LncR3. In addition, IDH1 mutation and 1p/19q LOH were found rich with LncR3, and EGFR amplification showed high percentage in LncR1 in GSE16011 dataset.
The researchers report a novel molecular classification of glioma based on lncRNA expression profiles and believe that it would provide a potential platform for future studies on gene treatment for glioma and lead to more individualized therapies to improve survival rates.
- Li R, Qian J, Wang YY, Zhang JX, You YP. (2014) Long Noncoding RNA Profiles Reveal Three Molecular Subtypes in Glioma. CNS Neurosci Ther [Epub ahead of print]. [abstract]
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