Tag Archives: lncrna

Knockdown of lncRNAs: exploring RNAi and antisense oligo methods

lncRNA

Thousands of different long non-coding RNAs (lncRNAs) exist in mammalian cells. lncRNAs do not encode proteins but can be very important for cell function. Studying their functions can be difficult because of their diverse modes of action. One method to discern cellular function is by selective knockdown of a specific lncRNA species. However, achieving consistent knockdown has proven to be ...

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Long non-coding RNA Databases in Cardiovascular Research

lncrna

With rising interest in the regulatory functions of long non-coding RNAs (lncRNAs) in complex human diseases such as cardiovascular diseases, there is an increasing need in public databases offering comprehensive and integrative data for all aspects of these versatile molecules. Recently, a variety of public data repositories specialized in lncRNAs have been developed, which make use of huge high-throughput data ...

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Long Non-Coding RNAs – Methods and Protocols

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The advent of next-generation sequencing revealed that the majority of the human genome is transcribed to noncoding RNAs. Recent studies on long noncoding RNAs (lncRNAs) suggest that they may represent another class of important, nonprotein regulators of various biological processes, including cell proliferation, differentiation, migration, apoptosis, and transformation. In this column of the Methods in Molecular Biology series, the authors ...

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Function of Mysterious RNAs May Often Lie in Their Genes

lncrna

Study co-led by Penn Medicine researcher shows non-coding DNA acting as enhancer of nearby gene’s expression PHILADELPHIA—A new genetic clue discovered by a team co-led by a researcher at the Perelman School of Medicine at the University of Pennsylvania is shedding light on the functions of the mysterious “long non-coding RNAs” (lncRNAs). These molecules are transcribed from genes and are ...

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Altered Long Noncoding RNA Expression Precedes the Course of Parkinson’s Disease

lncRNA

Parkinson’s disease (PD) is a slowly progressing neurodegenerative disorder that affects approximately seven million patients worldwide. Despite intensive research, the molecular mechanisms initiating and promoting PD are still unknown. However, it is assumed that environmental factors trigger PD. Recent research demonstrated that long noncoding RNAs (lncRNA) interfere in transcriptional and translational processes modulating gene expression reflecting environmental influences. Nevertheless, there ...

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DECKO – Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs

lncRNA

For some time, scientists have been seeking a more efficient and reliable way to edit the genome and modify it to suit any need. A technique called CRISPR-Cas9 was recently launched as the solution to this problem, and has since taken a position as one of the most revolutionary techniques in molecular biology. Although CRISPR-Cas9 is much more powerful than ...

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Long noncoding RNAs in lung cancer – what we know in 2015

lncRNA

Lung cancer ranks as the first most common cancer and the first leading cause of cancer-related death in China and worldwide. Due to the difficulty in early diagnosis and the onset of cancer metastasis, the 5-year survival rate of lung cancer remains extremely low. Long noncoding RNAs (lncRNAs), which lacking protein-coding ability, have recently emerged as pivotal participants in biological ...

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Symbiosis with Ancient Viruses Critical for Human Development

lncRNA

from Bioscience Technology by Cynthia Fox In recent years humans have come to understand we are not just about Darwinian natural selection, but symbiosis. For two billion years, there were only bacteria and archaea. Then a single archaea swallowed a bacteria in such a way the bacteria became its powerpack. Complex life exploded out of this symbiosis. Remnants of that ...

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Post-transcriptional regulation of long noncoding RNAs in cancer

lncRNA

It is a great surprise that the genomes of mammals and other eukaryotes harbor many thousands of long noncoding RNAs (lncRNAs). Although these long noncoding transcripts were once considered to be simply transcriptional noise or cloning artifacts, multiple studies have suggested that lncRNAs are emerging as new players in diverse human diseases, especially in cancer, and that the molecular mechanisms ...

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Community Curated Database For LncRNA

lncRNA

A wiki-style database hopes to serve as an online encyclopedia of lncRNA by and for the scientific community. Scientists have set up a long non-coding RNA (lncRNA) database aimed at harnessing the collective knowledge of the scientific community. This study has been published in Nucleic Acids Research. LncRNAs are RNAs that do not code for proteins but are nonetheless actively ...

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