Tag Archives: lncrna

LncRNAs and miRNAs – potential biomarkers and therapeutic targets for prostate cancer

Prostate cancer (PCa) is the second lethal disease for men in western countries. Although androgen receptor (AR) signaling has been widely investigated, noncoding RNAs (ncRNAs), deficient of open reading frame, have also received considerable attention. Growing studies showed that the aberrant ncRNAs expression contributed to cell proliferation, metastasis and drug resistance in PCa. Therefore, therapeutically targeting ncRNAs may synergize androgen ...

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Visualizing the secondary and tertiary architectural domains of lncRNA RepA

lncRNA

Long noncoding RNAs (lncRNAs) are important for gene expression, but little is known about their structures. RepA is a 1.6-kb mouse lncRNA comprising the same sequence as the 5′ region of Xist, including A and F repeats. It has been proposed to facilitate the initiation and spread of X-chromosome inactivation, although its exact role is poorly understood. To gain insight ...

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Logic programming to infer complex RNA expression patterns from RNA-seq data

lncRNA

To meet the increasing demand in the field, numerous long noncoding RNA (lncRNA) databases are available. Given many lncRNAs are specifically expressed in certain cell types and/or time-dependent manners, most lncRNA databases fall short of providing such profiles. Researchers at the University of Louisville have developed a strategy using logic programming to handle the complex organization of organs, their tissues ...

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UCSF CRISPR Study Reveals Unexpected Roles of Long Non-Coding RNAs

lncRNA

High-Throughput Screening Platform Finds that Distinct Non-Coding RNAs are Essential to the Growth of Different Cancer Cell Types UC San Francisco researchers have taken a major step toward understanding the function of the tens of thousands of human genes that do not code for proteins, a phenomenon considered one of the key remaining mysteries of the human genome. New findings ...

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Identification of a serum circulating lncRNA panel for the diagnosis and recurrence prediction of bladder cancer

Accumulating evidence indicates that long non-coding RNAs (lncRNAs) play important roles in tumorigenesis and progression. Researchers from Shandong University aimed to identify a panel of lncRNAs for the diagnosis and recurrence prediction in bladder cancer (BC). The expression of 13 candidate lncRNAs was investigated in 80 BC and matched adjacent normal tissues via quantitative real-time PCR. The differentially expressed lncRNAs ...

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Long intergenic non-coding RNA expression signature in human breast cancer

lncrna

Breast cancer is a complex disease, characterized by gene deregulation. There is less systematic investigation of the capacity of long intergenic non-coding RNAs (lincRNAs) as biomarkers associated with breast cancer pathogenesis or several clinicopathological variables including receptor status and patient survival. Researchers at the Vanderbilt University Medical Center designed a two-stage study, including 1,000 breast tumor RNA-seq data from The ...

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Structural and Functional Annotation of Long Noncoding RNAs

lncrna

Protein-coding RNAs represent only a small fraction of the transcriptional output in higher eukaryotes. The remaining RNA species encompass a broad range of molecular functions and regulatory roles, a consequence of the structural polyvalence of RNA polymers. Albeit several classes of small noncoding RNAs are relatively well characterized, the accessibility of affordable high-throughput sequencing is generating a wealth of novel, ...

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Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

lncRNA

RNA fluorescence in situ hybridization (FISH), long an indispensable tool for the detection and localization of RNA, is becoming an increasingly important complement to other gene expression analysis methods. Especially important for long noncoding RNAs (lncRNAs), RNA FISH adds the ability to distinguish between primary and mature lncRNA transcripts and thus to segregate the site of synthesis from the site ...

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SINEUP lncRNAs represent a new versatile tool to improve the production of secreted proteins in biomanufacturing processes

lncRNA

Mammalian cells are an indispensable tool for the production of recombinant proteins in contexts where function depends on post-translational modifications. Among them, Chinese Hamster Ovary (CHO) cells are the primary factories for the production of therapeutic proteins, including monoclonal antibodies (MAbs). To improve expression and stability, several methodologies have been adopted, including methods based on media formulation, selective pressure and ...

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Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) may function as a potential biomarker for glioma

lncRNA

Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, researchers from Nanchang University used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. They found that expression of CCAT2 was up-regulated in glioma ...

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