Tag Archives: Harvard University

Emerging mechanisms of long noncoding RNA function during normal and malignant hematopoiesis

lncRNA

Long noncoding RNAs (lncRNAs) are increasingly recognized as vital components of gene programs controlling cell differentiation and function. Central to their functions is an ability to act as scaffolds or as decoys that recruit or sequester effector proteins from their DNA, RNA, or protein targets. lncRNA-modulated effectors include regulators of transcription, chromatin organization, RNA processing, and translation, such that lncRNAs ...

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Chromatin environment, transcriptional regulation, and splicing distinguish lincRNAs and mRNAs

lncRNA

While long intergenic noncoding RNAs (lincRNAs) and mRNAs share similar biogenesis pathways, these transcript classes differ in many regards. LincRNAs are less evolutionarily conserved, less abundant, and more tissue-specific, suggesting that their pre- and post-transcriptional regulation is different from that of mRNAs. Here, researchers from Harvard University performed an in-depth characterization of the features that contribute to lincRNA regulation in ...

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Single molecule identification of homology-dependent interactions between long ssRNA and dsDNA

lncRNA

Long non-coding RNAs (lncRNAs) are prominently associated with chromosomes in an ever-increasing diversity of roles. To provide further insight into the potential nature of these associations, Harvard University researchers have explored, for the first time, the interaction of long single-stranded (ss) RNAs with cognate homologous double-stranded (ds) DNA in vitro Using magnetic tweezers, they measured the effects of ssRNA on ...

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“Cat’s Cradling” the 3D Genome by the Act of LncRNA Transcription

lncRNA

There is growing evidence that transcription and nuclear organization are tightly linked. Yet, whether transcription of thousands of long noncoding RNAs (lncRNAs) could play a role in this packaging process remains elusive. Although some lncRNAs have been found to have clear roles in nuclear architecture (e.g., FIRRE, NEAT1, XIST, and others), the vast majority remain poorly understood. In this Perspective, ...

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lincRNAs are required for life

lncRNA

Many studies are uncovering functional roles for long noncoding RNAs (lncRNAs), yet few have been tested for in vivo relevance through genetic ablation in animal models. To investigate the functional relevance of lncRNAs in various physiological conditions, a team led by researchers at Harvard University have developed a collection of 18 lncRNA knockout strains in which the locus is maintained transcriptionally ...

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Junking the idea of junk

from The Economist A newly recognised class of genes really does matter GENETICS often progresses by breaking things. Early experiments used naturally broken genes—mutations—to work out the basic rules. Then geneticists found out how to induce mutations with radiation and chemicals. That gave them more material to work with, but the mutations still appeared at random. Then the “knockout mouse” ...

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Featured long non-coding RNA – RMST

Long noncoding RNAs (lncRNAs) are abundant in the mammalian transcriptome, and many are specifically expressed in the brain. Researchers from the Genome Institute of Singapore and Harvard University have identified a group of lncRNAs, including rhabdomyosarcoma 2-associated transcript (RMST), which are indispensable for neurogenesis. Here, they provide mechanistic insight into the role of human RMST in modulating neurogenesis. RMST expression ...

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Ribosome profiling reveals resemblance between long non-coding RNAs and 5' leaders of coding RNAs

lncRNA

Large-scale genomics and computational approaches have identified thousands of putative long non-coding RNAs (lncRNAs). It has been controversial, however, as to what fraction of these RNAs is truly non-coding. Here, researchers from Harvard University and MIT combined ribosome profiling with a machine-learning approach to validate lncRNAs during zebrafish development in a high throughput manner. They found that dozens of proposed ...

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Presentation – REGULATION OF CHROMATIN STRUCTURE BY NONCODING RNA

DANESH MOAZED: “REGULATION OF CHROMATIN STRUCTURE BY NONCODING RNA” (CELLULAR DYNAMICS RESEARCH TALKS) Date/Time Tue, 4/9/2013, 11:45 a.m. – 1 p.m. Location Biolabs Lecture Hall 1080, 16 Divinity Ave. Description “Regulation of chromatin structure by noncoding RNA”Danesh Moazed, Ph.D. Professor of Cell Biology, Harvard Medical School HHMI Investigator https://moazed.med.harvard.edu/ Abstract: Noncoding RNAs play central roles in the assembly of repressive ...

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