Tag Archives: Harvard Medical School

Post-doc Position Available – Fibrosis and long non-coding RNAs

lncrna

A postdoctoral position is available for a highly-motivated candidate to study mechanisms of hepatic stellate cell biology and liver fibrosis in the Mullen Laboratory in the Gastrointestinal Unit at Massachusetts General Hospital and Harvard Medical School. The candidate will lead projects to understand how long noncoding RNAs and protein-coding genes regulate hepatic stellate activity and liver fibrosis using both tissue ...

Read More »

lncRNA TERRA RNA Antagonizes ATRX and Protects Telomeres

Through an integration of genomic and proteomic approaches to advance understanding of long noncoding RNAs, researchers from Harvard Medical School investigate the function of the telomeric transcript, TERRA. By identifying thousands of TERRA target sites in the mouse genome, they demonstrate that TERRA can bind both in cis to telomeres and in trans to genic targets. They then define a ...

Read More »

Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity

lncRNA

A combination of transcription factors, enhancers, and epigenetic marks determines the expression of the key transcription factor FoxP3 in regulatory T cells (Tregs). Adding an additional layer of complexity, the long noncoding RNA (lncRNA) Flicr (Foxp3 long intergenic noncoding RNA) is a negative regulator that tunes Foxp3 expression, resulting in a subset of Tregs with twofold- to fivefold-lower levels of ...

Read More »

DIGIT – a conserved lncRNA regulator of endoderm differentiation in embryonic stem cells

lncRNA

Long noncoding RNAs (lncRNAs) exhibit diverse functions, including regulation of development. Here, researchers from Massachusetts General Hospital combined genome-wide mapping of SMAD3 occupancy with expression analysis to identify lncRNAs induced by activin signaling during endoderm differentiation of human embryonic stem cells (hESCs). They found that DIGIT is divergent to Goosecoid (GSC) and expressed during endoderm differentiation. Deletion of the SMAD3-occupied enhancer ...

Read More »

LINC00520 plays a functional role in breast cancer

lncRNA

Long non-coding RNAs (lncRNAs) have been implicated in normal cellular homeostasis as well as pathophysiological conditions, including cancer. Harvard Medical School researchers performed global gene expression profiling of mammary epithelial cells transformed by oncogenic v-Src, and identified a large subset of uncharacterized lncRNAs potentially involved in breast cancer development. Specifically, their analysis revealed a novel lncRNA, LINC00520 that is upregulated ...

Read More »

Long non-coding RNAs link extracellular matrix gene expression to ischemic cardiomyopathy

lncRNA

Ischemic cardiomyopathy (ICM) resulting from myocardial infarction is a major cause of heart failure. Recently, thousands of long non-coding RNAs (lncRNAs) have been discovered and implicated in a variety of biological processes. However, the role of most lncRNAs in heart failure remains largely unknown. The aim of this study is to test the hypothesis that the expression and function of ...

Read More »

A Dis3l2-mediated quality control pathway for lncRNA RMRP and other noncoding RNAs

lncRNA

Mutations in the 3′-5′ exonuclease DIS3L2 are associated with Perlman syndrome and hypersusceptibility to Wilms tumorigenesis. Previously, Harvard Medical School researchers found that Dis3l2 specifically recognizes and degrades uridylated pre-let-7 microRNA. However, the widespread relevance of Dis3l2-mediated decay of uridylated substrates remains unknown. Here, the researchers applied an unbiased RNA immunoprecipitation strategy to identify Dis3l2 targets in mouse embryonic stem ...

Read More »

Featured lncRNA – DEANR1

lncRNA

Long non-coding RNAs (lncRNAs) regulate diverse biological processes, including cell lineage specification. Here, researchers from Harvard Medical School report transcriptome profiling of human endoderm and pancreatic cell lineages using purified cell populations. Analysis of the data sets allows us to identify hundreds of lncRNAs that exhibit differentiation-stage-specific expression patterns. As a first step in characterizing these lncRNAs, they focus on an ...

Read More »

High-resolution Xist binding maps reveal two-step spreading during X-chromosome inactivation

The Xist long noncoding RNA (lncRNA) is essential for X-chromosome inactivation (XCI), the process by which mammals compensate for unequal numbers of sex chromosomes1, 2, 3. During XCI, Xist coats the future inactive X chromosome (Xi)4 and recruits Polycomb repressive complex 2 (PRC2) to the X-inactivation centre (Xic)5. How Xist spreads silencing on a 150-megabases scale is unclear. Here a ...

Read More »