Tag Archives: gene expression

The multidimensional mechanisms of long noncoding RNA function

lncrna

A major shift in our understanding of genome regulation has emerged recently. It is now apparent that the majority of cellular transcripts do not code for proteins, and many of them are long noncoding RNAs (lncRNAs). Increasingly, studies suggest that lncRNAs regulate gene expression through diverse mechanisms. Researchers from the University of Navarra review emerging mechanistic views of lncRNAs in gene ...

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How do lncRNAs regulate transcription?

lncRNA

It has recently become apparent that RNA, itself the product of transcription, is a major regulator of the transcriptional process. In particular, long noncoding RNAs (lncRNAs), which are so numerous in eukaryotes, function in many cases as transcriptional regulators. These RNAs function through binding to histone-modifying complexes, to DNA binding proteins (including transcription factors), and even to RNA polymerase II. ...

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Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection

lncRNA

Long non-coding RNAs (lncRNAs) are rapidly emerging as important regulators of a diverse array of cellular functions. Here, Columbia University researchers describe a meta-analysis of two independent RNA-seq studies to identify lncRNAs that are differentially expressed upon HIV-1 infection. Only three lncRNA genes exhibited altered expression of ≥ 2-fold in HIV-1-infected cells. Of these, the uncharacterized lncRNA LINC00173 was chosen ...

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Post-doc and PhD job opportunities

lncRNA

Institute of Biochemistry and Biophysics PAS is looking for candidates for three Postdocs and 4 PhD students starting on 1 of October or as soon as possible thereafter. Szymon Swiezewski laboratory is interested in studying different aspects of seed dormancy regulator DOG1 gene, with an emphasis on novel gene expression regulatory mechanisms in plants. Our work has already helped to ...

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Genetic variation in long noncoding RNAs and the risk of nonalcoholic fatty liver disease

lncRNA

The human transcriptome comprises a myriad of non protein-coding RNA species, including long noncoding RNAs (lncRNAs), which have a remarkable role in transcriptional and epigenetic regulation. Researchers at the University of Buenos Aires hypothesized that variants in lncRNAs influence the susceptibility to nonalcoholic fatty liver disease (NAFLD). Using next generation sequencing, they performed a survey of genetic variation associated with randomly ...

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Logic programming to infer complex RNA expression patterns from RNA-seq data

lncRNA

To meet the increasing demand in the field, numerous long noncoding RNA (lncRNA) databases are available. Given many lncRNAs are specifically expressed in certain cell types and/or time-dependent manners, most lncRNA databases fall short of providing such profiles. Researchers at the University of Louisville have developed a strategy using logic programming to handle the complex organization of organs, their tissues ...

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Genome-wide changes in lncRNA, splicing, and regional gene expression patterns in autism

lncRNA

Autism spectrum disorder (ASD) involves substantial genetic contributions. These contributions are profoundly heterogeneous but may converge on common pathways that are not yet well understood. Here, through post-mortem genome-wide transcriptome analysis of the largest cohort of samples analysed so far, researchers from UCLA interrogate the noncoding transcriptome, alternative splicing, and upstream molecular regulators to broaden our understanding of molecular convergence ...

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Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

lncRNA

RNA fluorescence in situ hybridization (FISH), long an indispensable tool for the detection and localization of RNA, is becoming an increasingly important complement to other gene expression analysis methods. Especially important for long noncoding RNAs (lncRNAs), RNA FISH adds the ability to distinguish between primary and mature lncRNA transcripts and thus to segregate the site of synthesis from the site ...

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Long non-coding RNAs – spatial amplifiers that control nuclear structure and gene expression

Over the past decade, it has become clear that mammalian genomes encode thousands of long non-coding RNAs (lncRNAs), many of which are now implicated in diverse biological processes. Recent work studying the molecular mechanisms of several key examples – including Xist, which orchestrates X chromosome inactivation – has provided new insights into how lncRNAs can control cellular functions by acting ...

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lncRNAs LOC146880 and ENST00000439577 may promote the progression of NSCLC

lncRNA

To identify what long non-coding RNAs (lncRNAs) are involved in non-small cell lung cancer (NSCLC), researchers from the Shanghai Jiao Tong University School of Medicine analyzed microarray data on gene expression and methylation. Gene expression chip and HumanMethylation450BeadChip were used to interrogate genome-wide expression and methylation in tumor samples. Differential expression and methylation were analyzed through comparing tumors with adjacent ...

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