A significant proportion of mammalian genomes corresponds to genes that transcribe long non-coding RNAs (lncRNAs). Throughout the last decade, the number of studies concerning the roles played...
Read More »Nuclear long noncoding RNAs: key regulators of gene expression
A significant portion of the human genome encodes genes that transcribe long nonprotein-coding RNAs (lncRNAs). A large number of lncRNAs localize in the nucleus, either enriched on the chromatin...
Read More »The multidimensional mechanisms of long noncoding RNA function
A major shift in our understanding of genome regulation has emerged recently. It is now apparent that the majority of cellular transcripts do not code for proteins, and many of them are long noncoding RNAs (lncRNAs). Increasingly, studies suggest that lncRNAs regulate gene expression through diverse mechanisms. Researchers from the University of Navarra review emerging mechanistic views of lncRNAs in gene ...
Read More »How do lncRNAs regulate transcription?
It has recently become apparent that RNA, itself the product of transcription, is a major regulator of the transcriptional process. In particular, long noncoding RNAs (lncRNAs), which are so numerous in eukaryotes, function in many cases as transcriptional regulators. These RNAs function through binding to histone-modifying complexes, to DNA binding proteins (including transcription factors), and even to RNA polymerase II. ...
Read More »Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection
Long non-coding RNAs (lncRNAs) are rapidly emerging as important regulators of a diverse array of cellular functions. Here, Columbia University researchers describe a meta-analysis of two independent RNA-seq studies to identify lncRNAs that are differentially expressed upon HIV-1 infection. Only three lncRNA genes exhibited altered expression of ≥ 2-fold in HIV-1-infected cells. Of these, the uncharacterized lncRNA LINC00173 was chosen ...
Read More »Post-doc and PhD job opportunities
Institute of Biochemistry and Biophysics PAS is looking for candidates for three Postdocs and 4 PhD students starting on 1 of October or as soon as possible thereafter. Szymon Swiezewski laboratory is interested in studying different aspects of seed dormancy regulator DOG1 gene, with an emphasis on novel gene expression regulatory mechanisms in plants. Our work has already helped to ...
Read More »Genetic variation in long noncoding RNAs and the risk of nonalcoholic fatty liver disease
The human transcriptome comprises a myriad of non protein-coding RNA species, including long noncoding RNAs (lncRNAs), which have a remarkable role in transcriptional and epigenetic regulation. Researchers at the University of Buenos Aires hypothesized that variants in lncRNAs influence the susceptibility to nonalcoholic fatty liver disease (NAFLD). Using next generation sequencing, they performed a survey of genetic variation associated with randomly ...
Read More »Logic programming to infer complex RNA expression patterns from RNA-seq data
To meet the increasing demand in the field, numerous long noncoding RNA (lncRNA) databases are available. Given many lncRNAs are specifically expressed in certain cell types and/or time-dependent manners, most lncRNA databases fall short of providing such profiles. Researchers at the University of Louisville have developed a strategy using logic programming to handle the complex organization of organs, their tissues ...
Read More »Genome-wide changes in lncRNA, splicing, and regional gene expression patterns in autism
Autism spectrum disorder (ASD) involves substantial genetic contributions. These contributions are profoundly heterogeneous but may converge on common pathways that are not yet well understood. Here, through post-mortem genome-wide transcriptome analysis of the largest cohort of samples analysed so far, researchers from UCLA interrogate the noncoding transcriptome, alternative splicing, and upstream molecular regulators to broaden our understanding of molecular convergence ...
Read More »Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells
RNA fluorescence in situ hybridization (FISH), long an indispensable tool for the detection and localization of RNA, is becoming an increasingly important complement to other gene expression analysis methods. Especially important for long noncoding RNAs (lncRNAs), RNA FISH adds the ability to distinguish between primary and mature lncRNA transcripts and thus to segregate the site of synthesis from the site ...
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