Tag Archives: CRISPR

Researchers develop new approach to study long non-coding RNAs

lncrna

New method identifies and determines role for newly discovered genetic units Until recently, scientific research concentrated almost exclusively on the 2 percent of the genome’s protein coding regions, virtually ignoring the other 98 percent – a vast universe of non-coding genetic material previously dismissed as ‘junk.’ In the past 10 years, scientists have discovered thousands of new long non-coding RNAs ...

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Researchers use CRISPR to show that the short splicing isoform of Xist RNA is sufficient to induce X-chromosome inactivation

lncRNA

Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and short isoforms of Xist RNA. Although a differentiation-specific and a female-specific ...

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Post-doc position available to study novel lncRNAs

lncRNA

A post-doctoral position is available immediately to study novel lncRNAs in a well-funded laboratory at the University of Rochester School of Medicine and Dentistry.  The laboratory was the first to report on a human-specific lncRNA (SENCR) enriched in vascular cells and has been a leading cardiovascular lab in CRISPR genome editing.  The lab uses a broad palette of tools to ...

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Researchers develop new CRISPR sgRNA design tool, demonstrate efficient CRISPR deletion of an enhancer and exonic fragment of the lncRNA MALAT1

lncRNA

CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements. However, no solution has been presented for the unique bioinformatic design requirements of CRISPR deletion. Researchers from the Centre for ...

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CRISPR-Mediated Interference (CRISPRi) of Long Non-Coding RNA (lncRNA)

lncRNA

by Jerry Zon More Methodology from CRISPR Mania lncRNA Function Blocked by CRISPRi Mysteries of lncRNA Can Now be Deciphered by CRISPRi This blog is about yet another example of a powerful new methodology spawned by intense scientific interest in using CRISPR-related technologies. This near mania for all things CRISPR is reflected by there being ~5,000 (!) publications already in ...

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Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

lncRNA

Long non-coding RNAs (lncRNAs) represent a large subgroup of RNAs that are over 200 nucleotides long and have a limited protein-coding potential. They play an important role in diverse cellular processes by regulating the gene expression at transcriptional or post-transcriptional levels. This ability of lncRNAs to modulate gene expression renders them indispensable for normal development CRISPRs (Clustered Regularly Interspaced Palindromic ...

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Effective knockdown of Drosophila long noncoding RNAs by CRISPR interference.

lncRNA

Ji-Long Liu (Univ Oxford) presents ‘Effective knockdown of Drosophila long noncoding RNAs by CRISPR interference.’ A presentation at the ‘Techniques & Resources’ session of the Annual Drosophila Research Conference at The Allied Genetics Conference, July 13-17, 2016, Orlando, Florida. Read the abstract: http://www.genetics-gsa.org/genetics/… For more presentations from #TAGC16 visit: http://www.genetics-gsa.org/tagcvideos or browse the playlists at: https://www.youtube.com/user/Genetics… More on #TAGC16: http://www.genetics2016.org/ ...

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UCSF CRISPR Study Reveals Unexpected Roles of Long Non-Coding RNAs

lncRNA

High-Throughput Screening Platform Finds that Distinct Non-Coding RNAs are Essential to the Growth of Different Cancer Cell Types UC San Francisco researchers have taken a major step toward understanding the function of the tens of thousands of human genes that do not code for proteins, a phenomenon considered one of the key remaining mysteries of the human genome. New findings ...

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CRISPR Deletion-Based Screen Identifies Functional Long Non-Coding RNAs

lncRNA

from GenomeWeb by Andrew Han – A new study published today in Nature Biotechnology turns high-throughput CRISPR/Cas9-based knockout screening towards long non-coding RNAs to find functional elements. A Chinese-American collaboration, led by first author Shiyou Zhu and senior author Wei Li  of Peking University and first author Wensheng Wei and senior author Xiaole Shirley Liu of the Dana-Farber Cancer Institute, ...

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Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

lncRNA

The CRISPR/Cas9 system provides a revolutionary genome editing tool for all areas of molecular biology. In long non-coding RNA (lncRNA) research, the Cas9 nuclease can delete lncRNA genes or introduce RNA-destabilizing elements into their locus. The nuclease-deficient dCas9 mutant retains its RNA-dependent DNA-binding activity and can modulate gene expression when fused to transcriptional repressor or activator domains. Here, researchers from ...

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