Tag Archives: alternative splicing

Researchers use CRISPR to show that the short splicing isoform of Xist RNA is sufficient to induce X-chromosome inactivation

lncRNA

Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and short isoforms of Xist RNA. Although a differentiation-specific and a female-specific ...

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Researchers identify cancer-related long noncoding RNAs in quintuple-negative lung adenocarcinoma

lncRNA

Lung adenocarcinoma (LUAD) is a common subtype of non-small cell lung cancer prevalent in Asia. There is a dearth of understanding regarding the transcriptome landscape of LUAD without primary known driver mutations. In this study, researchers at MSD China R&D used LUAD samples without well-known driver mutations occurring in EGFR, KRAS, ALK, ROS1 or RET (quintuple-negative) for transcriptome study with ...

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Functions of long intergenic non-coding (linc) RNAs in plants

lncRNA

Whole transcriptome analyses in many organisms have revealed that most transcribed RNAs do not encode proteins. These non-coding RNAs likely contribute to the regulation of gene expression during the development of multicellular organisms. In eukaryotes, the roles of small RNAs, one class of non-coding RNAs, in transcriptional and post-transcriptional regulation have been well characterized. However, the functions of a second ...

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Insertion of an Alu element in a lncRNA leads to primate-specific modulation of alternative splicing

lncRNA

Noncoding RNAs, mobile elements, and alternative splicing are all critical for the regulation of gene expression. Here researchers from the University of Science and Technology of China show that a conserved noncoding RNA acquires a new function due to the insertion of a mobile element. They identified a noncoding RNA, termed 5S-OT, which is transcribed from 5S rDNA loci in ...

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RACE-Seq – extension of human lncRNA transcripts by RACE coupled with long-read high-throughput sequencing

rna-seq

Long non-coding RNAs (lncRNAs) constitute a large, yet mostly uncharacterized fraction of the mammalian transcriptome. Such characterization requires a comprehensive, high-quality annotation of their gene structure and boundaries, which is currently lacking. Here researchers from the Barcelona Institute of Science and Technology describe RACE-Seq, an experimental workflow designed to address this based on RACE (rapid amplification of cDNA ends) and ...

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Long noncoding RNAs and prostate carcinogenesis: the missing 'linc'?

Long noncoding RNAs (lncRNAs) are rapidly becoming essential pieces in the cancer puzzle. Our understanding of their functional capabilities is in its infancy. One certain fact, however, is that their molecular interactions extend beyond chromatin complexes into diverse biological processes. In prostate cancer, aberrant expression of lncRNAs is associated with disease progression. Overexpression of oncogenic lncRNAs promotes tumor-cell proliferation and ...

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Biological and bioinformatical approaches to study crosstalk of long-non-coding RNAs

Long-non-coding RNA (lncRNA) regulates gene expression through transcriptional and epigenetic regulation as well as alternative splicing in the nucleus. In addition, regulation is achieved at the levels of mRNA translation, storage and degradation in the cytoplasm. During recent years, several studies have described the interaction of lncRNAs with enzymes that confer so-called epigenetic modifications, such as DNA methylation, histone modifications ...

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Species-specific alternative splicing leads to unique expression of sno-lncRNAs

Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented. By exploring non-polyadenylated transcriptomes from human, rhesus and mouse, researchers at the Shanghai Institutes for Biological Sciences have systematically annotated ...

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Species-specific alternative splicing leads to unique expression of sno-lncRNAs

Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented. By exploring non-polyadenylated transcriptomes from human, rhesus and mouse, researchers at the Shanghai Institutes for Biological Sciences have systematically annotated ...

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Species-specific alternative splicing leads to unique expression of sno-lncRNAs

Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented. By exploring non-polyadenylated transcriptomes from human, rhesus and mouse, we have systematically annotated sno-lncRNAs expressed in all three species. In ...

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