Search Results for: incrna
Many studies are uncovering functional roles for long noncoding RNAs (lncRNAs), yet few have been tested for in vivo relevance through genetic ablation in animal models. To investigate the functional relevance of lncRNAs in various physiological conditions, a team led by researchers at Harvard University have developed a collection of 18 lncRNA knockout strains in which the locus is maintained transcriptionally active. Initial characterization revealed peri- and postnatal lethal phenotypes in three mutant strains (Fendrr, Peril, and Mdgt), the latter two exhibiting incomplete penetrance and growth defects in survivors. The team also report growth defects for two additional mutant strains (linc-Brn1b and linc-Pint). Further analysis revealed defects in lung, gastrointestinal tract, and heart in Fendrr(-/-) neonates, whereas linc-Brn1b(-/-) mutants displayed distinct abnormalities in the generation of upper layer II-IV neurons in the neocortex. This study demonstrates that lncRNAs play critical roles in vivo and provides a framework and impetus for future larger-scale functional investigation into the roles of lncRNA molecules.
- Sauvageau M et al. (2013) Multiple knockout mouse models reveal lincRNAs are required for life and brain development. Elife 2(0), e01749. [article]
Incoming search terms:
- incRNA blog
- lncRNA chip
- peril and genecard
- what is function of incrna
An inducible program of inflammatory gene expression is central to antimicrobial defenses. Signal-dependent activation of transcription factors, transcriptional co-regulators, and chromatin modifying factors collaborate to control this response. Here, researchers from University of Massachusetts Medical School identify a long noncoding RNA that acts as a key regulator of this inflammatory response. Pattern recognition receptors such as the Toll-like receptors induce the expression of numerous lncRNAs. One of these, lincRNA-Cox2 mediates both the activation and repression of distinct classes of immune genes. Transcriptional repression of target genes is dependent on interactions of lincRNA-Cox2 with heterogeneous nuclear ribonucleoprotein A/B and A2/B1. Collectively, these studies unveil a central role of lincRNA-Cox2 as a broad acting regulatory component of the circuit that controls the inflammatory response.
- Carpenter S, Atianand M, Aiello D, Ricci EP, Gandhi P, Hall LL, Byron M, Monks B, Henry-Bezy M, Lawrence JB, O’Neill LA, Moore MJ, Caffrey DR, Fitzgerald KA. (2013) A Long Noncoding RNA Mediates Both Activation and Repression of Immune Response Genes. Science [Epub ahead of print]. [abstract]
Incoming search terms:
- long non cording RNA Cox2
- lincrna cox2 human
- lncRNA COX2
- long noncoding RNA influenza virus
The function of the non-coding portion of the human genome remains one of the most important questions of our time. Its vast complexity is exemplified by the recent identification of an unusual and notable component of the transcriptome – very long intergenic non-coding RNAs, termed vlincRNAs.
Here a team led by researchers from the St. Laurent Institute identify 2,147 vlincRNAs covering 10 percent of our genome. They show they are present not only in cancerous cells, but also in primary cells and normal human tissues, and are controlled by canonical promoters. Furthermore, vlincRNA promoters frequently originate from within endogenous retroviral sequences. Strikingly, the number of vlincRNAs expressed from endogenous retroviral promoters strongly correlates with pluripotency or the degree of malignant transformation. These results suggest a previously unknown connection between the pluripotent state and cancer via retroviral repeat-driven expression of vlincRNAs. Finally, they show that vlincRNAs can be syntenically conserved in humans and mouse and their depletion using RNAi can cause apoptosis in cancerous cells.
- St Laurent G 3rd, Shtokalo D, Dong B, Tackett MR, Fan X, Lazorthes S, Nicolas E, Sang N, Triche TJ, McCaffrey TA, Xiao W, Kapranov P. (2013) VlincRNAs controlled by retroviral elements are a hallmark of pluripotency and cancer. Genome Biol 14(7), R73. [abstract]
Incoming search terms:
- st laurent institute
Press Release: Six Carnegie Mellon Professors Awarded Research Grants From Pittsburgh Foundation’s Charles E. Kaufman Fund
PITTSBURGH—Six Carnegie Mellon University professors are among the first series of grant recipients of The Charles E. Kaufman Foundation, part of The Pittsburgh Foundation, which today announced nearly $1.6 million in research grants to support cutting-edge scientific research at institutions across Pennsylvania.
Carnegie Mellon recipients are:
- Assistant Professor of Biological Sciences Joel McManus for research on “High-Throughput Probing of Human IncRNA Structure.”
- Assistant Professor of Chemical Engineering Aditya S. Khair for research on “Charges, Forces and Particles in Ionic Liquids.”
- Associate Professor of Biological Sciences Veronica Hinman, Professor of Biological Sciences Jonathan Minden, Chemistry Professor Bruce Alan Armitage and Associate Chemistry Professor Danith H. Ly for research on “Developing a Sea Star Model for Regenerative Biology.”
McManus and Khair will receive two-year, $150,000 New Investigator grants. Hinman, Minden, Armitage and Ly will receive a two-year, $300,000 New Initiative grant.