lncRNA-HIT promotes cell proliferation of non-small cell lung cancer

Lung cancer is the leading cause of cancer-related death around the world. Long noncoding RNA (lncRNA) has pivotal roles in cancer occurrence and development. However, only a few lncRNAs have been functionally characterized. Jilin University researchers investigated the effects of lncRNA-HIT (HOXA transcript induced by TGFβ) expression on non-small cell lung cancer (NSCLC) cell phenotype with the gain-of-function and loss-of-function assays. They found that ectopic expression or knockdown of lncRNA-HIT markedly increased or decreased NSCLC cell proliferation, respectively. Moreover, they also showed that lncRNA-HIT interacted with E2F1 to regulate its target genes, such as Survivin, FOXM1, SKP2, NELL2 and DOK1. Collectively, these findings indicated that lncRNA-HIT affected the proliferation of NSCLC cells at least in part via regulating the occupancy of E2F1 in the promoter regions of its target genes. The lncRNA-HIT-E2F1 complex may be a potential target for NSCLC treatment.

lncRNA-HIT promotes cell proliferation in NSCLC cells

lncRNA

(a) qPCR analysis of the RNA level of lncRNA-HIT in cells transfected with control siRNA or lncRNA-HIT siRNAs. (b) Cells with transfection of control siRNA or lncRNA-HIT siRNAs were examined by colony formation assay. (c) Cells with transfection of control siRNA or lncRNA-HIT siRNAs were examined by the CCK-8 assay. (d) qPCR analysis of the RNA level of lncRNA-HIT in cells with or without transfection of lncRNA-HIT. (e) Cells with or without transfection of lncRNA-HIT were examined by colony formation assay. (f) Cells with or without transfection of lncRNA-HIT were examined by the CCK-8 assay. Data are shown as mean±s.d. *P<0.05. lncRNA, long noncoding RNA; NSCLC, non-small cell lung cancer; qPCR, quantitative real-time PCR; siRNA, short interfering RNA.

Yu L, Fang F, Lu S, Li X, Yang Y, Wang Z. (2017) lncRNA-HIT promotes cell proliferation of non-small cell lung cancer by association with E2F1. Cancer Gene Ther [Epub ahead of print]. [abstract]

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