Long non-coding RNAs (lncRNA) which are longer than 200 base pairs in length, play important role in the cellular machinery. Chronic lymphocytic leukemia (CLL) and multiple myeloma (MM) are neoplasms of B-cells.
Researchers at Istanbul University aimed to investigate circulating lncRNA levels of CLL and MM patients. For this purpose they selected 5 candidate lncRNAs (TUG1, LincRNA-p21, MALAT1, HOTAIR, and GAS5) where the first two are regulated by p53. Analyses were performed by real-time PCR using cDNA synthesized from plasma RNAs. In both disease groups differential levels of plasma lncRNAs were observed. LincRNA-p21 was the only molecule displaying significant changes in the CLL group while all remaining lncRNAs showed significant differences in the MM group. In the MM group only TUG1 showed higher levels than the healthy volunteers. In conclusion, the expression levels of the candidate lncRNA molecules display a general trend for tissue- and disease-specific expression which can provide important potential biomarkers specific to the particular disease type.
- Isin M, Ozgur E, Cetin G, Erten N, Aktan M, Gezer U, Dalay N. (2014) Investigation of Circulating LncRNAs in B-cell Neoplasms. Clin Chim Acta [Epub ahead of print]. [abstract]