Identification of an lncRNA‑miRNA‑mRNA interaction mechanism in breast cancer

Non‑coding RNAs serve important roles in regulating the expression of certain genes and are involved in the principal biological processes of breast cancer. The majority of studies have focused on defining the regulatory functions of long non‑coding RNAs (lncRNAs) and microRNAs (miRNAs/miRs), and few studies have investigated how lncRNAs and miRNAs are transcriptionally regulated.

In the present study, based on the breast invasive carcinoma dataset from The Cancer Genome Atlas at cBioPortal, and using a bioinformatics computational approach, researchers at Zhejiang Sci‑Tech University constructed an lncRNA‑miRNA‑mRNA network. The network consisted of 601 nodes and 706 edges, which represented the complex web of regulatory effects between lncRNAs, miRNAs and target genes. The results of the present study demonstrated that miR‑510 was the most potent miRNA controller and regulator of numerous target genes. In addition, it was observed that the lncRNAs PVT1, CCAT1 and linc00861 exhibited possible interactions with clinical biomarkers, including receptor tyrosine‑protein kinase erbB‑2, estrogen receptor and progesterone receptor, demonstrated using RNA‑protein interaction prediction software. The network of lncRNA‑miRNA‑mRNA interactions will facilitate further experimental studies and may be used to refine biomarker predictions for developing novel therapeutic approaches in breast cancer.

RNA fold reliability data of probable long non‑coding RNA‑microRNA pairs

 lncRNA

(A) CCAT1‑hsa‑miR‑1178. (B) PVT1‑hsa‑miR‑5196. (C) linc00861‑hsa‑miR‑510 (D) linc00861‑hsa‑miR‑140‑3p. linc, long intervening non‑coding; miR, microRNA.

ZhangY, Li Y, Wang Q, Zhang X, Wang D, Tang HC, Meng X, Ding X. (2017) Identification of an lncRNA‑miRNA‑mRNA interaction mechanism in breast cancer based on bioinformatic analysis. Molecular Medicine Reports [Epub ahead of print]. [abstract]

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