Targeted Neuroblastoma Therapy One Step Closer

from ScienceNetwork WA by  Anika Rodgers AUSTRALIAN researchers have uncovered new targets for therapy in the childhood cancer neuroblastoma. Histone More »

Newly Discovered Large Molecule Could be Key to Treating Heart Failure

from Bioresearch Online by C. Rajan, contributing writer Researchers at the Indiana University School of Medicine have just discovered a More »

lncRNA Market Trends – Learn the latest about the expanding and evolving long noncoding RNA space.

from Genetic Engineering News by Enal Razvi, Ph.D. The focus of this GEN Market & Tech Analysis report is to More »

Epigenetic coordination of embryonic heart transcription by dynamically regulated long noncoding RNAs

The role of noncoding RNAs in mammalian biology is of great interest, especially since the Encyclopedia of DNA Elements results More »

The Long Noncoding RNA Expression Profile of Hepatocellular Carcinoma Identified by Microarray Analysis

Thousands of long noncoding RNAs (lncRNAs) have been reported in mammalian genomes. These RNAs represent an important subset of pervasive More »

 

Review Paper

Long non-coding RNAs in cancer: implications for personalized therapy

Long non-coding RNAs (lncRNAs, pseudogenes and circRNAs) have recently come into light as powerful players in cancer pathogenesis and it is becoming increasingly clear that they have the potential of greatly contributing to the spread and success of personalized cancer medicine. In this concise review, the authors briefly introduce these three classes of long non-coding RNAs. We then discuss their applications as diagnostic and prognostic biomarkers. Finally, we describe their appeal as targets and as drugs, while pointing out the limitations that still lie ahead of their definitive entry into clinical practice.

lncRNA

  • Vitiello M, Tuccoli A, Poliseno L. (2014) Long non-coding RNAs in cancer: implications for personalized therapy. Cell Oncol (Dordr). [Epub ahead of print]. [abstract]

Non-coding RNA – Zooming in on lncRNA functions

lncRNALong non-coding RNAs (lncRNAs) are emerging as important regulators of gene expression; however, in contrast to transcription factors, their functional domain architecture remains poorly understood. Now, a new method has been developed to simultaneously map RNA–RNA, RNA–DNA and RNA–protein interactions at the level of individual RNA domains with increased sensitivity.

Quinn et al. developed a method called domain-specific chromatin isolation by RNA purification (dChIRP), in which several antisense oligonucleotide pools are used to target specific domains of lncRNAs. In this method, cells are subjected to fixation, crosslinking and sonication, and the resultant sheared chromatin is hybridized to the biotinylated oligonucleotide pools to recover chromatin fragments containing specific lncRNA domains of interest. The RNA, DNA and protein components associated with the lncRNA domains can then be analysed separately.

LncRNA: A link between RNA and cancer

Unraveling the gene expression networks governing cancer initiation and development is essential while remains largely uncompleted. With the innovations in RNA-seq technologies and computational biology, long noncoding RNAs (lncRNAs) are being identified and characterized at a rapid pace. Recent findings reveal that lncRNAs are implicated in serial steps of cancer development. These lncRNAs interact with DNA, RNA, protein molecules and/or their combinations, acting as an essential regulator in chromatin organization, transcriptional and post-transcriptional regulation. Their misexpression confers the cancer cell capacities for tumor initiation, growth, and metastasis. The review here will emphasize their aberrant expression and function in cancer, and the roles in cancer diagnosis and therapy will be also discussed.

lncRNA

  • Yanga G, Lub X, Yuanc L. (2014) LncRNA: A link between RNA and cancer. Biochimica et Biophysica Acta (BBA) – Gene Regulatory Mechanisms [Epub ahead of print]. [abstract]

Incoming search terms:

  • lncrna p53 interaction
  • lncRNA-DNA

Long non-coding RNAs in cancer invasion and metastasis

Recent large-scale transcriptome analyses have revealed that the human genome contains more than just protein-coding genes. Indeed, a large number of transcripts, including long non-coding RNAs (lncRNAs), lack protein-coding capacity, and increasing evidence suggests that lncRNAs could have a critical role in the regulation of diverse cellular processes, such as stem cell pluripotency, development, cell growth and apoptosis, and cancer invasion and/or metastasis. Furthermore, the aberrant expression of several lncRNAs is closely linked to cancer invasion and/or metastasis. Although the underlying molecular mechanisms by which lncRNAs regulate cancer invasion and/or metastasis are not clearly understood, recent studies have revealed that aberrant lncRNAs expression affects the progression of cancer. In this review, we highlight recent findings regarding the roles of lncRNAs in cancer invasion and/or metastasis.

lncRNA

  • Shen XH, Qi P, Du X. (2014) Long non-coding RNAs in cancer invasion and metastasis. Mod Pathol [Epub ahead of print]. [abstract]

Incoming search terms:

  • apoptosis postdoctoral position 2014 august

Long Noncoding RNAs in Imprinting and X Chromosome Inactivation

The field of long noncoding RNA (lncRNA) research has been rapidly advancing in recent years. Technological advancements and deep-sequencing of the transcriptome have facilitated the identification of numerous new lncRNAs, many with unusual properties, however, the function of most of these molecules is still largely unknown. Some evidence suggests that several of these lncRNAs may regulate their own transcription in cis, and that of nearby genes, by recruiting remodeling factors to local chromatin. Notably, lncRNAs are known to exist at many imprinted gene clusters. Genomic imprinting is a complex and highly regulated process resulting in the monoallelic silencing of certain genes, based on the parent-of-origin of the allele. It is thought that lncRNAs may regulate many imprinted loci, however, the mechanism by which they exert such influence is poorly understood. This review will discuss what is known about the lncRNAs of major imprinted loci, and the roles they play in the regulation of imprinting.

lncRNA

  • Autuoro JM, Pirnie SP, Carmichael GG. (2014) Long Noncoding RNAs in Imprinting and X Chromosome Inactivation. Biomolecules 4(1):76-100. [article]

Incoming search terms:

  • xact x inactivation
  • high-resolution xist binding maps reveal two-step spreading during x-chromosome inactivation
  • human chromosome lncRNA
  • mechanism of action of non coding RNAs in health
  • noncoding region of X chromosome
  • [x chromosome inactivation in human: xact and xist a non coding rna for each x]