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Genetic variation in long noncoding RNAs and the risk of nonalcoholic fatty liver disease

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The human transcriptome comprises a myriad of non protein-coding RNA species, including long noncoding RNAs (lncRNAs), which have a remarkable role in transcriptional and epigenetic regulation. Researchers at the University of Buenos Aires hypothesized that variants in lncRNAs influence the susceptibility to nonalcoholic fatty liver disease (NAFLD). Using next generation sequencing, they performed a survey of genetic variation associated with randomly ...

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Researchers at Imperial College London have found that so-called ‘junk DNA’ could play a role in diabetes

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The human genome is enormous, containing billions of ‘letters’ of genetic code. But among the thousands of genes which code for vital proteins, hidden in plain sight are much vaster chunks of non-coding junk DNA, previously thought to have no function. Shining the spotlight on these dark, unexplored regions of the genome, an international team led by scientists at Imperial ...

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The short ASCC3 RNA isoform functions as a long non-coding RNA

Researchers from the Francis Crick Institute analyzed the transcription-related DNA damage response on a genome-wide scale with great spatial and temporal resolution. Upon UV irradiation, a slowdown of transcript elongation and restriction of gene activity to the promoter-proximal ∼25 kb is observed. This is associated with a shift from expression of long mRNAs to shorter isoforms, incorporating alternative last exons ...

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CRISPR-Mediated Interference (CRISPRi) of Long Non-Coding RNA (lncRNA)

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by Jerry Zon More Methodology from CRISPR Mania lncRNA Function Blocked by CRISPRi Mysteries of lncRNA Can Now be Deciphered by CRISPRi This blog is about yet another example of a powerful new methodology spawned by intense scientific interest in using CRISPR-related technologies. This near mania for all things CRISPR is reflected by there being ~5,000 (!) publications already in ...

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lncRNA co-expression network model for the prognostic analysis of acute myeloid leukemia

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Acute myeloid leukemia (AML) is a highly heterogeneous hematologic malignancy with great variability of prognostic behaviors. Previous studies have reported that long non-coding RNAs (lncRNAs) play an important role in AML and may thus be used as potential prognostic biomarkers. However, thus use of lncRNAs as prognostic biomarkers in AML and their detailed mechanisms of action in this disease have ...

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Featured lncRNA Job – Technical Scientist

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Position available One opening for either a Technical Scientist or Technical Staff Job description We are seeking a highly motivated individual to elucidate the molecular function of long non-coding RNA based on functional genomics strategy (e.g. high-throughput screening). The ideal candidate should have a strong background in managing and executing automation system such as automated cell culturing system and automated ...

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A panel of four-long noncoding RNA signatures predicts prostate cancer survival

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Growing evidences showed that lncRNAs abnormally expressed in cancer tissues and played irreplaceable roles in tumorigenesis, progression and metastasis. In present study, researchers from Yangzhou University aimed to identify lncRNA expression signature that can predict biochemical recurrence-free (BCR-free) survival of prostate cancer (PCa) patients. A total of 291 patients with pathologic confirmed PCa in The Cancer Genome Atlas dataset were ...

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In Silico identification and annotation of non-coding RNAs by RNA-seq and De Novo assembly of the transcriptome

The complexity of the tomato (Solanum lycopersicum) transcriptome has not yet been fully elucidated. To gain insights into the diversity and features of coding and non-coding RNA molecules of tomato fruits, researchers from the University of Naples Federico II generated strand-specific libraries from berries of two tomato cultivars grown in two open-field conditions with different soil type. Following high-throughput Illumina RNA-sequencing ...

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Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

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Long non-coding RNAs (lncRNAs) represent a large subgroup of RNAs that are over 200 nucleotides long and have a limited protein-coding potential. They play an important role in diverse cellular processes by regulating the gene expression at transcriptional or post-transcriptional levels. This ability of lncRNAs to modulate gene expression renders them indispensable for normal development CRISPRs (Clustered Regularly Interspaced Palindromic ...

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