Alzheimer’s Disease Model Displays Distinct Hippocampal Expression Profiles of Long Non-coding RNAs

Alzheimer’s disease (AD), the most prevalent form of dementia worldwide, is a complex neurodegenerative disease characterized by the progressive loss of memory and other cognitive functions. The pathogenesis of AD is not yet completely understood. Although long non-coding RNAs (lncRNAs) have recently been shown to play a role in AD pathogenesis, the specific influences of lncRNAs in AD remain largely unknown; in particular, hippocampal lncRNA expression profiles in AD rats are lacking.

In this study, microarray analysis was performed to investigate the hippocampal expression patterns of dysregulated lncRNAs in a rat model of AD. A total of 315 lncRNAs and 311 mRNAs were found to be significantly dysregulated in the AD model (≥2.0 fold, p < 0.05). Then, quantitative real-time PCR was used to validate the expression of selected lncRNAs and mRNAs. Bioinformatics tools and databases were employed to explore the potential lncRNA functions.

The heat map and hierarchical clustering of the top 40 differentially expressed lncRNAs

lncRNA

(a) and mRNAs (b) between AD and control hippocampal samples. The data are depicted as a data matrix, in which each row represents one lncRNA (mRNA) and each column represents one of the hippocampal samples. The relative lncRNA (mRNA) expression is depicted according to the colour scale shown at the top. Red represents high relative expression, and green represents low relative expression; −2.0, 0 and 2.0 are FCs in the corresponding spectrum. The magnitude of deviation from the median is represented by the colour saturation

This is the first study to comprehensively identify dysregulated hippocampal lncRNAs in AD and to demonstrate the involvement of different lncRNA expression patterns in the hippocampal pathogenesis of AD. This information will enable further research on the pathogenesis of AD and facilitate the development of novel AD therapeutics targeting lncRNAs.

Yang B, Xia ZA, Zhong B, Xiong X, Sheng C, Wang Y, Gong W, Cao Y, Wang Z, Peng W. (2016) Distinct Hippocampal Expression Profiles of Long Non-coding RNAs in an Alzheimer’s Disease Model. Mol Neurobiol [Epub ahead of print]. [article]

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