Long non-coding RNAs: evolution of new epigenetic and post-transcriptional functions

9:00 am on Monday 28 September 2015 — 5:00 pm on Tuesday 29 September 2015 at The Royal Society at More »

Featured long non-coding RNA – meiRNA

Long non-coding RNAs (lncRNAs) play key roles in the formation of nuclear bodies. In the fission yeast Schizosaccharomyces pombe, a More »

Lengthy RNAs earn respect as cellular players

from Science by Elizabeth Pennisi Surveys have uncovered thousands of long non-coding RNAs, molecules longer than 200 bases, and several More »

The RIDL hypothesis: transposable elements as functional domains of long noncoding RNAs

Our genome contains tens of thousands of long noncoding RNAs (lncRNAs), many of which are likely to have genetic regulatory More »

Featured lncRNA – ncRuPAR

ncRuPAR is a newly discovered long noncoding RNA molecule that can upregulate protease-activated receptor-1 (PAR-1) during embryonic growth; however, its More »

 

Profiling Long Intergenic Non-Coding RNA Interactions In The Cancer Genome

Profiling Long Intergenic Non-Coding RNA Interactions In The Cancer Genome – Samir Amin

May 12, 2014 – The Cancer Genome Atlas 3rd Annual Scientific Symposium
More: http://www.genome.gov/27557040

Featured Long Non-Coding RNA – BANCR

Long non-coding RNAs (lncRNAs) have been shown to be implicated in the complex network of cancer including malignant melanoma and play important roles in tumorigenesis and progression. However, their functions and downstream mechanisms are largely unknown.

banThis study aimed to investigate whether BRAF-activated non-coding RNA (BANCR), a novel and potential regulator of melanoma cell, participates in the proliferation of malignant melanoma and elucidate the underlying mechanism in this process. Researchers from the Inner Mongolia People’s Hospital found that BANCR was abnormally overexpressed in human malignant melanoma cell lines and tissues, and increased with tumor stages by quantitative PCR. BANCR knockdown induced by shRNA transfection significantly inhibited proliferation of tumor cells and inactivated MAPK pathway, especially by silencing the ERK1/2 and JNK component. Moreover, combination treatment of BANCR knockdown and suppression ERK1/2 or JNK (induced by specific inhibitors U0126 or SP600125 respectively) produced synergistic inhibitory effects in vitro. And the inhibitory effects induced by ERK1/2 or JNK could be rescued by BANCR overexpression. By tumorigenicity assay in BALB/c nude mice, we further found that BANCR knockdown inhibited tumor growth in vivo. In addition, patients with high expression of BANCR had a lower survival rate. Taken together, we confirmed the abnormal upregulation of a novel lncRNA, BANCR, in human malignant melanoma. BANCR was involved in melanoma cell proliferation both in vitro and in vivo. The linkage between BANCR and MAPK pathway may provide a novel interpretation for the mechanism of proliferation regulation in malignant melanoma.

  • Li R, Zhang L, Jia L, Duan Y, Li Y, et al. (2014) Long Non-Coding RNA BANCR Promotes Proliferation in Malignant Melanoma by Regulating MAPK Pathway Activation. PLoS ONE 9(6): e100893. [article]

Long Noncoding RNAs in Imprinting and X Chromosome Inactivation

The field of long noncoding RNA (lncRNA) research has been rapidly advancing in recent years. Technological advancements and deep-sequencing of the transcriptome have facilitated the identification of numerous new lncRNAs, many with unusual properties, however, the function of most of these molecules is still largely unknown. Some evidence suggests that several of these lncRNAs may regulate their own transcription in cis, and that of nearby genes, by recruiting remodeling factors to local chromatin. Notably, lncRNAs are known to exist at many imprinted gene clusters. Genomic imprinting is a complex and highly regulated process resulting in the monoallelic silencing of certain genes, based on the parent-of-origin of the allele. It is thought that lncRNAs may regulate many imprinted loci, however, the mechanism by which they exert such influence is poorly understood. This review will discuss what is known about the lncRNAs of major imprinted loci, and the roles they play in the regulation of imprinting.

lncRNA

  • Autuoro JM, Pirnie SP, Carmichael GG. (2014) Long Noncoding RNAs in Imprinting and X Chromosome Inactivation. Biomolecules 4(1):76-100. [article]

Long non-coding RNAs: evolution of new epigenetic and post-transcriptional functions

lncRNA

Theo Murphy scientific meeting organised by Professor Leonard Lipovich, Professor John Rinn, Professor Douglas Higgs FRS, Professor Nicholas Proudfoot FRS and Professor Lynne Maquat.

Event details

The discovery that long non-protein-coding RNAs (lncRNAs) are prevalent in metazoan transcriptomes has been a highlight of the second post-genomic decade. Unexpectedly, lncRNAs mediate remarkably diverse cellular mechanisms. This meeting will synthesise insights that derive from next-generation sequencing, functional genomics, and lncRNA structure and function to augment our understanding of how lncRNAs contribute to species uniqueness and to human disease.

The draft programme, and abstracts and biographies of the speakers will be made available shortly. Recorded audio of the presentations will be available on this page after the event.

Attending this event

This is a residential conference, which allows for increased discussion and networking. It is free to attend, however participants need to cover their accommodation and catering costs if required.

Enquiries: Contact the events team

Long noncoding RNAs and prostate carcinogenesis: the missing ‘linc’?

Long noncoding RNAs (lncRNAs) are rapidly becoming essential pieces in the cancer puzzle. Our understanding of their functional capabilities is in its infancy. One certain fact, however, is that their molecular interactions extend beyond chromatin complexes into diverse biological processes. In prostate cancer, aberrant expression of lncRNAs is associated with disease progression. Overexpression of oncogenic lncRNAs promotes tumor-cell proliferation and metastasis through chromatin looping and distal engagement with the androgen receptor, antisense gene regulation, alternative splicing, and impeding DNA repair. Several lncRNAs, such as prostate cancer antigen 3 (PCA3), prostate cancer gene expression marker 1 (PCGEM1), and prostate cancer associated ncRNA transcript 1 (PCAT1), are highly prostate-specific, posing as attractive biomarkers.

lncRNA

  • Walsh AL, Tuzova AV, Bolton EM, Lynch TH, Perry AS. (2014) Long noncoding RNAs and prostate carcinogenesis: the missing ‘linc’? Trends Mol Med [Epub ahead of print]. [abstract]