Neuroblastoma is an embryonal tumor of the sympathetic nervous system and the most common extracranial tumor of childhood. By sequencing More »
Long non-coding RNAs (lncRNAs, pseudogenes and circRNAs) have recently come into light as powerful players in cancer pathogenesis and it More »
The study analysed experiments carried out on six different species and identified almost 2,500 IncRNAs that were not in the More »
from Bioresearch Online by C. Rajan, contributing writer Researchers at the Indiana University School of Medicine have just discovered a More »
Neuroblastoma is an embryonal tumor of the sympathetic nervous system and the most common extracranial tumor of childhood. By sequencing transcriptomes of low- and high-risk neuroblastomas, we detected differentially expressed annotated and nonannotated long noncoding RNAs (lncRNAs). We identified a lncRNA neuroblastoma associated transcript-1 (NBAT-1) as a biomarker significantly predicting clinical outcome of neuroblastoma. CpG methylation and a high-risk neuroblastoma associated SNP on chromosome 6p22 functionally contribute to NBAT-1 differential expression. Loss of NBAT-1 increases cellular proliferation and invasion. It controls these processes via epigenetic silencing of target genes. NBAT-1 loss affects neuronal differentiation through activation of the neuronal-specific transcription factor NRSF/REST. Thus, loss of NBAT-1 contributes to aggressive neuroblastoma by increasing proliferation and impairing differentiation of neuronal precursors.
- Gaurav Kumar Pandey, Sanhita Mitra, Santhilal Subhash, Falk Hertwig, Meena Kanduri, Kankadeb Mishra, Susanne Fransson, Abiarchana Ganeshram, Tanmoy Mondal, Sashidhar Bandaru, Malin Östensson, Levent M. Akyürek, Jonas Abrahamsson, Susan Pfeifer, Erik Larsson, Leming Shi, Zhiyu Peng, Matthias Fischer, Tommy Martinsson, Fredrik Hedborg, Per Kogner, Chandrasekhar Kanduri. (2014) The Risk-Associated Long Noncoding RNA NBAT-1 Controls Neuroblastoma Progression by Regulating Cell Proliferation and Neuronal Differentiation. Cancer Cell 26(5): 722–737. [abstract]
Script that finds likely mirna primary transcripts in gencode data by seeing which long non-coding RNAs have miRNA overlapping them.
Long non-coding RNAs (lncRNAs) are non-protein coding transcripts longer than 200 nucleotides. The post-transcriptional regulation is influenced by these lncRNAs by interfering with the microRNA pathways, involving in diverse cellular processes. The regulation of gene expression by lncRNAs at the epigenetic level, transcriptional and post-transcriptional level have been well known and widely studied. Recent recognition that lncRNAs make effects in many biological and pathological processes such as stem cell pluripotency, neurogenesis, oncogenesis and etc. This review focuses on the functional roles of lncRNAs in epigenetics and related research progress are summarized.
- Cao J. (2014) The functional role of long non-coding RNAs and epigenetics. Biol Proced Online 16:11. [article]
|Tuesday, October 14, 2014|
|University of Virginia School of Medicine, Charlottesville, EUA|
SEMINAR | 14 OCTOBER | 12:00 | MAIN AUDITORIUM
Small and long noncoding RNAs in control of cell proliferation and differentiation
Anindya Dutta, University of Virginia School of Medicine, Charlottesville, EUA
Long non-coding RNAs (lncRNAs, pseudogenes and circRNAs) have recently come into light as powerful players in cancer pathogenesis and it is becoming increasingly clear that they have the potential of greatly contributing to the spread and success of personalized cancer medicine. In this concise review, the authors briefly:
- Introduce these three classes of long non-coding RNAs.
- Discuss their applications as diagnostic and prognostic biomarkers.
- Describe their appeal as targets and as drugs,
- Point out the limitations that still lie ahead of their definitive entry into clinical practice.
- Vitiello M, Tuccoli A, Poliseno L. (2014) Long non-coding RNAs in cancer: implications for personalized therapy. Cell Oncol (Dordr) [Epub ahead of print]. [abstract]